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Figure 4 | Lipids in Health and Disease

Figure 4

From: Immunohistochemical localization of hepatopancreatic phospholipase in gastropods mollusc, Littorina littorea and Buccinum undatum digestive cells

Figure 4

Section of digestive gland showing mSDPLA2 immunoreactivity in digestive tubules and immunofluorescence localization of phospholipase in a 4-μm frozen section. (A and C) control satining in absence of primary antiserum. (B, D) Few mSDPLA2 positive nuclei were detected. These immunoreactive nuclei correspond mainly to digestive cells (arrows). Basophilic cells remained unstained (D). (E) One section was first stained with DAPI for general morphology. (F) pAbs anti mSDPL (1:100) revealed by fluorochrome (chromeo™488) conjugated to secondary antibody diluted 1:200. (G and H) merge pictures of (E and F), showing labeling of intracellular granules of digestive cells. Sections of digestive glands showed immunoreactivity to mSDPLA2 immunoreactivity in digestive tubules. Few mSDPLA2 positive nuclei appeared.

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