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Table 1 Composition of control and TFA-enriched diets according to AIN-93

From: Hydrogenated fat intake during pregnancy and lactation caused increase in TRAF-6 and reduced AdipoR1 in white adipose tissue, but not in muscle of 21 days old offspring rats

Ingredient Diet (g/100 g)
  C T
Casein* 20 20
L-cystine 0.3 0.3
Cornstarch 62 62
Soybean oil 8 1
Hydrogenated vegetable fat§ - 7
Butyl hydroquinone 0.0014 0.0014
Mineral mixture 3.5 3.5
Vitamin mixture 1.0 1.0
Cellulose 5.0 5.0
Choline bitartrate 0.25 0.25
Energy (kcal/g) 4.0 4.0
  1. C, control; T, hydrogenated vegetable fat; TFA, trans-fatty acid
  2. * Casein was obtained from CoperLab, São Paulo, Brazil.
  3. L-cystine, cornstarch, butyl hydroquinone, cellulose and choline bitartrate were obtained from Viafarma, São Paulo, Brazil.
  4. Oil was supplied from soybean (Lisa/Ind. Brazil).
  5. § Hydrogenated vegetable fat was supplied from Unilever, São Paulo, Brazil.
  6. || Mineral mix provided (mg/kg) calcium 5000, phosphorus 1561, potassium 3600, sodium 1019, chloride 1571, sulfur 300, magnesium 507, iron 35, copper 6.0, manganese 10.0, zinc 30.0, chromium 1.0, iodine 0.2, selenium 0.15, fluoride 1.00, boron 0.50, molybdenum 0.15, silicon 5.0, nickel 0.5, lithium 0.1, vanadium 0.1 (AIN-93G mineral mix DYETS 210025, Dyets Inc., Bethlehem, PA, USA).
  7. Vitamin mix (mg/kg diet) provided thiamin HCL 6.0, riboflavin 6.0, pyridoxine HCL 7.0, niacin 30.0, calcium pantothenate 16.0, folic acid 2.0, biotin 0.2, vitamin B12 25.0, vitamin A palmitate 4000 IU, vitamin E acetate 75, vitamin D3 1000 IU, vitamin KI 0.75 (AIN-93G, vitamin mix, DYETS 310025, Dyets Inc.).