Hydrolysis rate of TC
by SL1 as function of substrate concentration. The TC3 solutions were systematically prepared by mixing (3 × 30 s in warring blender) a given amount of TC3 in 30 mL of 0.3% GA and 0.15 M NaCl. The release of propionic acid was recorded continuously at pH 7 and 37°C using a pH-stat. The CMC of TC3 (12 mM) is indicated by vertical dotted lines.