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Figure 1 | Lipids in Health and Disease

Figure 1

From: 13-hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages

Figure 1

Effects of 13-HODE, LA and WY-14,643 on PPAR/PPRE transactivation activity and PPAR protein levels in RAW264.6 macrophages. A, RAW264.7 cells were transiently transfected with 3 × ACO-PPRE reporter vector. After transfection, cells were treated or not with 0.1-2.5 μmol/L 13-HODE, 1-100 μmol/L LA and 50 μmol/L WY-14,643 for 24 h. Afterwards, cells were lysed, and luciferase activities of the ACO-PPRE firefly luciferase vector and a co-transfected renilla luciferase vector determined by a dual luciferase assay. Bars represent means ± SD from four independent experiments (n = 4). Data are expressed as percentage of relative luciferase activity of vehicle control cells. Results from statistical analysis are indicated: Significant effects are denoted with an asterisk (P < 0.05). B, RAW264.7 cells were treated with 2.5 μmol/L 13-HODE, 100 μmol/L LA or vehicle (ethanol) for 24 h. Afterwards, cells were lysed and subsequently processed for western blotting as described in the materials and methods section. Representative immunoblots specific for PPARα, PPARγ, and β-actin which was used for normalization are shown. Bars represent data from densitometric analysis and are means ± SD from three independent experiments (n = 3). Data are expressed as percentage of protein concentration of vehicle control cells.

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Lipids in Health and Disease

ISSN: 1476-511X