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Figure 3 | Lipids in Health and Disease

Figure 3

From: 13-hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages

Figure 3

Effects of 13-HODE and LA on cholesterol concentrations in cells and medium of macrophages in macrophages in the presence and absence of apoA-I and PPARα and PPARγ antagonists. After pre-treatment without or with PPAR antagonists for 4 h and treatment of RAW264.7 macrophage cells without (vehicle control) or with 2.5 μmol/L 13-HODE or 100 μmol/L LA for 20 h, cells were incubated again without or with the antagonists for 4 h and afterwards without or with the corresponding fatty acids in the presence or absence of apolipoprotein A-I (apoA-I) (30 μg/mL) for 24 h. Afterwards, medium was collected, and cells were washed with PBS. Total lipids were extracted from medium and cells and concentrations of cholesterol determined as described in the materials and methods section. A, Cellular and B, medium cholesterol concentrations were related to cellular protein content. Bars represent means ± SD from four independent experiments (n = 4). Data are expressed as percentage of cholesterol concentration in cells and medium of control cells. Results from statistical analysis are indicated: Significant effects are denoted with an asterisk (P < 0.05).

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Lipids in Health and Disease

ISSN: 1476-511X