Figure 3
Effects of dietary supplementation with various compounds on the production of nitric oxide (μM) by LPS-stimulated thioglycolate-elicited peritoneal macrophages. 4-week-old and 42-week-old C57BL/6 male mice were fed control diet or control diets supplemented with quercetin, δ-tocotrienol or dexamethasone for 4 week. After this, thioglycolate-elicited peritoneal macrophages were collected, adhered to the bottom of 12 well plates (1×107 cells/well in 1.0 mL medium) for 4 h, then washed with medium three times. Cells were then treated with LPS (10 ng/well or 100 ng/well) for 18 h at room temperature, the mixtures were centrifuged at 2000 rpm for 20 min, and supernatants were collected, and stored at -70°C for subsequent analysis of NO using Griess reagent [12]. Cell viability exceeded 95% in all experiments [13]. Data represent means ± SD; n = 3 mice per group with triplicate analysis of each sample. Values in columns with different symbols differ at P< 0.05. Treatments 1 - 16 correspond to: 1 - 4 = 8-week-old or 5 - 8 = 46-week-old mice treated with 10 ng/well LPS; 9 - 12 = 8-week-old or 13 - 16 = 46-week-old mice treated with 100 ng/well LPS. CD = control diet; Quer = quercetin; Trie = δ-tocotrienol; Dexa = dexamethasone.