Figure 7
Effects of dietary supplementation of various compounds on the gene expression of iNOS and TNF-α in LPS-stimulated thioglycolate-elicited peritoneal macrophages derived from 8-week-old and 46-week-old C57BL/6 male mice after feeding for 4 weeks. 4-week-old and 42-week-old C57BL/6 male mice were fed control diet or control diet supplemented with quercetin, δ-tocotrienol or dexamethasone for 4 week. After this, thioglycolate-elicited peritoneal macrophages were collected, adhered to the bottom of 12 well plates (1×107 cells/well in 1.0 mL medium) for 4 h, then washed with medium three times. The cells were treated with LPS (10.0 ng/well) for 4 h, and the purified RNA of each mouse was subjected to RT-PCR analyses, as described in the experimental section. The top rows in Figure 7, correspond to β-Actin, middle rows, iNOS and bottom rows, TNF-α (8-week-old mice, 1-12, and 46-week-old mice, 13 - 24).8-week-old mice, for iNOS (middle row, 1-12), and TNF-α (bottom row, 1-12):1. Medium + macrophages = cells, 2. Cells + LPS 10 ng/well, 3. Cells + LPS 1 ng/well; 4. Medium + macrophages = cells, 5. Cells + LPS 10 ng/well + quercetin, 6. Cells + LPS 1 ng/well + quercetin; 7. Medium + macrophages = cells, 8. Cells + LPS 10 ng/well + δ-tocotrienol, 9. Cells + LPS 1 ng/well + δ-tocotrienol; 10. Medium + macrophages = cells, 11. Cells + LPS 10 ng/well + dexamethasone 12. Cells + LPS 1 ng/well + dexamethasone.46-week-old mice, for iNOS (second row, 13-24), and TNF-α (third row, 13-24):13. Medium + macrophages = cells, 14. Cells + LPS 10 ng/well, 15. Cells + LPS 1 ng/well; 16. Medium + macrophages = cells, 17. Cells + LPS 10 ng/well + quercetin, 18. Cells + LPS 1 ng/well + quercetin; 19. Medium + macrophages = cells, 20. Cells + LPS 10 ng/well + δ-tocotrienol, 21. Cells + LPS 1 ng/well + δ-tocotrienol; 22. Medium + macrophages = cells, 23. Cells + LPS 10 ng/well + dexamethasone 24. Cells + LPS 1 ng/well + dexamethasone.