Lipids in Health and Disease

Table 3 Effects of various compounds on the gene expression of TNF-α and iNOS in LPS-stimulated thioglycolate-elicited peritoneal macrophages derived from 8-week-old and 46-week-old C57BL/6 male mice after feeding for 4 weeks¹.

From: Inhibition of nitric oxide in LPS-stimulated macrophages of young and senescent mice by δ-tocotrienol and quercetin

8-week-old vs 46-week-old		RT-PCR (8-wk-old) data²		RT-PCR (46-wk-old) data²
NO		Gene Expression		Gene Expression
	C57BL/6 Male Mice	TNF-α	iNOS	TNF-α	iNOS
1	Media + Macrophages (MM)	0	0	0	0
2	MM + LPS, 10.0 ng/mL (A)	0.35 (100)*³	0.41 (100)³	0.38 (100)³	0.69 (100)³
3	A + Quercetin (100 ppm)	0.26 (74)	0.29 (71)	0.29 (76)	0.47 (68)
4	A + δ-Tocotrienol (100 ppm)	0.24 (69)	0.22 (54)	0.20 (53)	0.28 (41)
5	A + Dexamethasone (10 ppm)	0.20 (57)	0.15 (37)	0.27 (71)	0.20 (29)
	*Control groups	8-week-old vs	46-week-old
	TNF-α	0.35 (100)	0.38 (109)
	iNOS	0.41 (100)	0.69 (168)

¹The assay mixture (A; contains 0.2% dimethyl sulfoxide = DMSO) was treated with LPS (10.0 ng/well) for 4 h.
²Total RNA was extracted using RNAeasy mini Kit.
³Percentage of control ratio, based on MM + LPS 10.0 ng/well.

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ISSN: 1476-511X

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