Inhibition of nitric oxide in LPS-stimulated macrophages of young and senescent mice by δ-tocotrienol and quercetin

Table 4 Effects of LPS, LPS + quercetin, and quercetin on the levels of gene expression, and biological functions in macrophages of8-week-old C57BL/6 male mice¹

			Regulation		Regulation			Regulation
#	Functions	LPS	Up	Down	LPS + Quer	Up	Down	Quer	Up	Down
1	Immune response	182	130	52	124	84	40	39	9	30
2	Cell to cell signaling	124	86	38	136	78	58	41	4	37
3	Cellular growth & proliferation	74	54	20	99	61	38	36	22	14
4	Inflammatory diseases	74	53	21	88	61	27	24	1	23
5	Respiratory diseases	44	34	10	42	31	11	27	4	23
6	Immunological diseases	39	35	4	37	26	11	9	6	3
7	Cancer	34	25	9	42	28	14	13	9	4
8	Hepatic system diseases	26	23	3	5	5
9	Cardiovascular system	18	14	4	44	33	11	10	8	2
10	Carbohydrate Metabolism	8	5	3	13	1	12
11	Lipid metabolism	7	7		53	36	17	39	24	15
12	Neurological Diseases	6	5	1	34	29	5	4	4
13	Free-radical scavenging	5	4	1				4	3	1
14	Gene expression	4	4		7	6	1
Total		645	479	166	724	479	245	246	94	152

LPS = Lipopolysaccharide; Quer = Quercetin.
¹The data in this Table represents the expression of a number of genes modulated by various treatments; The macrophages were treated with LPS alone (10 ng/well), LPS (10 ng/well) + quercetin (40 μM), or quercetin (40 μM) alone as described in the experimental section. Total RNA was extracted from the treated cells and their gene expression profiles compared with the control (LPS), and untreated cells. The values here been corrected for differences in the arrays. The gene expression values are reported as average normalization log ratios. A data containing gene identifiers and their corresponding expression values were uploaded as an Excel spreadsheet using the template provided in the Ingenuity Pathways Analysis program. Similar conditions were used for data provided in Tables 8, 9, and 10.