Inhibition of nitric oxide in LPS-stimulated macrophages of young and senescent mice by δ-tocotrienol and quercetin

Table 5 Effects of LPS, LPS + δ-tocotrienol, and δ-tocotrienol on the levels of gene expression, and biological functions in macrophages of 8-week-old C57BL/6 male mice¹.

			Regulation		Regulation			Regulation
#	Functions	LPS	Up	Down	LPS + Trie	Up	Down	Trie	Up	Down
1	Immune response	182	130	52	132	80	52	50	40	10
2	Cell to cell signaling	124	86	38	97	46	51	52	40	12
3	Cellular growth & proliferation	74	54	20	55	42	13	32	18	14
4	Inflammatory diseases	74	53	21	9	5	4	5	5
5	Respiratory diseases	44	36	8				5	5
6	Immunological diseases	39	33	6	10	6	4	4	1	3
7	Cancer	34	25	9	29	22	7	17	12	5
8	Hepatic system diseases	26	23	3	8	7	1	3	2	1
9	Cardiovascular system	18	14	4	33	27	6	6	4	2
10	Carbohydrate Metabolism	8	5	3	14	5	9
11	Lipid metabolism	7	7		45	35	10	49	34	15
12	Neurological Diseases	6	5	1	26	24	2	4	4
13	Free-radical scavenging	5	4	1				4	3	1
14	Gene expression	4	4		23	19	4	3	3
Total		645	479	166	481	318	163	234	171	63

LPS = Lipopolysaccharide; Trie = δ-Tocotrienol.
¹The data in this Table represents the expression of a number of genes modulated by various treatments; macrophages were treated with LPS alone (10 ng/well), LPS (10 ng/well) + δ-tocotrienol (20 μM), or δ-tocotrienol (20 μM) alone as described in the experimental section. Total RNA was extracted from the treated cells and their gene expression profiles compared with the control (LPS), and untreated cells. The values here have been corrected for differences in the arrays. The gene expression values are reported as average normalization log ratios. A data set containing gene identifiers and their corresponding expression values were uploaded as an Excel spreadsheet using the template provided in Ingenuity Pathways Analysis Program. Similar conditions were used for data provided in Tables 8, 9, 10.

ISSN: 1476-511X