Skip to main content
Figure 4 | Lipids in Health and Disease

Figure 4

From: Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey

Figure 4

Purification steps of rTPL. (A) Chromatography of rTPL on Sephacryl S200; the column (2.5 × 150 cm) was equilibrated with 25 mM Tris buffer, pH 8.2, containing 25 mM NaCl and 2 mM Benzamidine (buffer B). The elution of proteins was performed with the same buffer at a rate of 30 ml/h. (B) Chromatography of rTPL on FPLC Mono-Q Sepharose. The column was equilibrated with buffer B; a linear gradient was applied from 25 to 350 mM NaCl in buffer B; the flow rate used was 2 ml/min. (C) Chromatography of rTPL on Sephadex G100; the column was equilibrated in buffer B and the flow rate was 25 ml/h. The pooled fractions containing the rTPL activity were indicated by a dashed line. (D) SDS-PAGE analysis of rTPL performed on 12% gel. Lane1: Low molecular weight marker; Lane 2: culture supernatant; Lane 3: active fraction from Sephacryl-S200; Lane 4: active fraction from Q-Sepharose FF; Lane 5: active fraction from Sephadex-G100.

Back to article page