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Figure 3 | Lipids in Health and Disease

Figure 3

From: Purification and biochemical characterization of a secreted group IIA chicken intestinal phospholipase A2

Figure 3

Effect of Ca2+ and NaTDC on ChPLA2-IB and ChPLA2-IIA activities. (A) Effect of Ca2+ concentration on ChPLA2-IIA (black circle) and ChPLA2-IB (white circle) activities. Enzyme activity was measured at various concentrations of Ca2+ using egg yolk emulsion as substrate at pH 9.0 and at 40°C in the presence of 10 mM NaTDC. The star indicates the phospholipase activity measured in the absence of CaCl2 and in the presence of 10 mM EDTA or EGTA. (B) Effect of increasing concentration of bile salts (NaTDC) on ChPLA2-IIA (black circle) and ChPLA2-IB (white circle) activities. PLA2 activity was measured using egg yolk emulsion as substrate at pH 9.0 and at 40°C in the presence of 10 mM Ca2+.

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