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Figure 4 | Lipids in Health and Disease

Figure 4

From: Purification and biochemical characterization of a secreted group IIA chicken intestinal phospholipase A2

Figure 4

Effect of temperature and pH on ChPLA2-IB and ChPLA2-IIA. Effects of temperature (A, B) and pH (C, D) on ChPLA2-IIA (black circle) and ChPLA2-IB (white circle) activities (A, C) and stability (B, D). PLA2 were tested for activity at various temperatures (A) and pH (C) as described in material and methods. To study the stability of PLA2, 1 mg.ml-1 of each enzyme was incubated during 30 min at various temperatures (B) and pH (D). Residual PLA2 activity was measured using egg yolk as substrate in the presence of 10 mM Ca2+ and 10 mM NaTDC. For temperature stability studies, enzymes were incubated in 10 mM Tris (pH 8.0) and 10 mM CaCl2. For pH stability studies, Tris buffer was replaced by the appropriate buffer for the pH range.

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