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Table 1 Flow sheet of chicken intestinal phospholipase A2 purification

From: Purification and biochemical characterization of a secreted group IIA chicken intestinal phospholipase A2

Purification Step

Total Activity (U)a

Total Protein (mg)b

Yield (%)

Specific activity (U/mg)

Purification Factor

Extraction

(pH 8.5)

250

5520

100

0.045

1

Acidic treatment (pH3)

123

205.5

49

0.6

13.33

(NH 4 ) 2 SO 4

Precipitation

(60%)

108

108

43

1

22.22

Sephadex G-50

95

6.41

38

14.8

328.88

MonoS Sepharose

25

0.156

10

160

3555

  1. a) 1 Unit: μmole of fatty acid released per min using egg yolk as substrate in the presence of 10 mM NaTDC and in the presence of 10 mM CaCl2.
  2. b) Proteins were estimated by Bradford method [30]. The experiments were conducted in triplicate.
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Lipids in Health and Disease

ISSN: 1476-511X