Table 1 Primers for real-time quantitative PCR analysis
From: Induction of heme oxygenase-1 protects against nutritional fibrosing steatohepatitis in mice
Gene | Product length | Primer sequences |
|---|---|---|
HO-1 | 427bp | F 5'-AACAAGCAGAACCCAGTCTATG-3' |
| Â | Â | R 5'-TGAGCAGGAAGGCGGTCTTA-3' |
TNF-α | 300bp | F 5'-GGCAGGTCTACTTTGGAGTCATTGC-3' |
| Â | Â | R 5'-ACATTCGAGGCTCCAGTGAATTCGG-3' |
IL-6 | 159bp | F 5'-AGT TGC CTT CTT GGG ACT GA-3' |
| Â | Â | R 5'-TCC ACG ATT TCC CAG AGA AC-3' |
α-SMA | 160bp | F 5'-CTGACAGAGGCACCACTGAA-3' |
| Â | Â | R 5'-CATCTCCAGAGTCCAGCACA-3' |
TGF-β1 | 272bp | F 5'-CAACGCCATCTATGAGAAAACC-3' |
| Â | Â | R 5'-ACTGCCGTACAACTCCAGTGAC-3' |
MMP-2 | 203bp | F 5'-CACACCAGGTGAAGGATGTG-3' |
| Â | Â | R 5'-AGGGCTGCATTGCAAATATC-3' |
MMP-9 | 67bp | F 5'-CAGTATCTGTATGGTCGTGGCT-3' |
| Â | Â | R 5'-TTCAGTTGTGGTGGTGGCT-3' |
SOCS1 | 123bp | F 5'-TCCTCGTCCTCGTCTTCGT-3' |
| Â | Â | R 5'-TAATCGGAGTGGGAGCGGA-3' |
GAPDH | 450bp | F 5'-ACCACAGTCCATGCCATCAC-3' |
| Â | Â | R 5'-TCCACCACCCTGTTGCTG-3' |