Skip to main content

Table 1 Primers for real-time quantitative PCR analysis

From: Induction of heme oxygenase-1 protects against nutritional fibrosing steatohepatitis in mice

Gene Product length Primer sequences
HO-1 427bp F 5'-AACAAGCAGAACCCAGTCTATG-3'
   R 5'-TGAGCAGGAAGGCGGTCTTA-3'
TNF-α 300bp F 5'-GGCAGGTCTACTTTGGAGTCATTGC-3'
   R 5'-ACATTCGAGGCTCCAGTGAATTCGG-3'
IL-6 159bp F 5'-AGT TGC CTT CTT GGG ACT GA-3'
   R 5'-TCC ACG ATT TCC CAG AGA AC-3'
α-SMA 160bp F 5'-CTGACAGAGGCACCACTGAA-3'
   R 5'-CATCTCCAGAGTCCAGCACA-3'
TGF-β1 272bp F 5'-CAACGCCATCTATGAGAAAACC-3'
   R 5'-ACTGCCGTACAACTCCAGTGAC-3'
MMP-2 203bp F 5'-CACACCAGGTGAAGGATGTG-3'
   R 5'-AGGGCTGCATTGCAAATATC-3'
MMP-9 67bp F 5'-CAGTATCTGTATGGTCGTGGCT-3'
   R 5'-TTCAGTTGTGGTGGTGGCT-3'
SOCS1 123bp F 5'-TCCTCGTCCTCGTCTTCGT-3'
   R 5'-TAATCGGAGTGGGAGCGGA-3'
GAPDH 450bp F 5'-ACCACAGTCCATGCCATCAC-3'
   R 5'-TCCACCACCCTGTTGCTG-3'
  1. Abbreviations: HO-1, heme oxygenase-1; TNF-α, tumor necrosis factor-alpha; IL-6, interleukin-6; α-SMA, α-smooth muscle actin; TGF-β1, transforming growth factor beta 1; MMP-2, matrix metallopeptidase-2; MMP-9, matrix metallopeptidase-9; SOCS1, suppressor of cytokine signaling-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.