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Figure 3 | Lipids in Health and Disease

Figure 3

From: Purification and biochemical characterization of pancreatic phospholipase A2 from the common stingray Dasyatis pastinaca

Figure 3

Effect of temperature and pH. (A) Effect of temperature on SPLA2, OPLA2 and DrPLA2 activity. (B) Effect of temperature on SPLA2, OPLA2 and DrPLA2 stability. The enzyme activity was tested at various temperatures using PC as substrate in the presence of 10 mM Ca 2+ and 4 mM NaDC and at standard conditions after incubation at 70°C at different times. (C) Effects of pH on SPLA2, OPLA2 and DrPLA2 activity and stability. (D) Effects of pH on SPLA2, OPLA2 and DrPLA2 activity and stability. The enzyme activity was tested at various pH using PC as substrate in the presence of 10 mM Ca2+ and 4 mM NaDC and at standard conditions after incubation at different pH. All experiments were repeated at least three times. SPLA2 (black circle); DrPLA2 (black square); OPLA2 (open square).

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Lipids in Health and Disease

ISSN: 1476-511X