Figure 2

In vitro bactericidal activity of chicken sPLA2-IIA. Samples of gram negative bacterial suspensions were taken after incubation with sPLA2 at various concentrations for 20, 60 and 120 min and thereafter cultured on agar. Bacterial viability was assessed by measuring colony forming ability as described in "experimental procedures". The results shown in the figure are means of two independent tests. The sPLA2 final concentrations were follows: white circle = 15 μg/ml sPLA2-IIA, white triangle = 30 μg/ml sPLA2-IIA, white square = 45 μg/ml sPLA2-IIA, black square = 50 μg/ml sPLA2-IB.