OA modulates vascular SMC phenotype. (A) Immunofluorescence staining of SMαA and SMemb proteins in rat aortic SMCs after incubation for 48 hours with vehicle (control), OA (250 μmol/L), or OA (250 μmol/L) plus SSO (250 μmol/L). Magnification ×400. (B, C) Summarized data showing the average fluorescence intensity in cells from each group. Data are expressed as mean ± SEM. ** p < 0.01 compared with the control group, # p < 0.05 compared with the cells treated with OA alone. OA, oleic acid; SMCs, smooth muscle cells; SSO, sulfosuccinimidyl oleate; SMαA, smooth muscle α-actin; SMemb, embryonic smooth muscle myosin heavy chain.