Construction of SR-PSOX fragment into pEGFP-N3 expression vector. Constructed products were analysed by 1% agarose gel to confirm the right size of fragment. Lane 1, empty pEGFP-N3 vector only (4700bp). Lane 2, wild type of SR-PSOX fragment was inserted in to the vector as pEGFP-N3-SR-PSOX construct (5541bp). Lane 3, pEGFP-N3-SR-PSOX construct was digested with EcoR I and BamH I to form the right size of two bands (4700bp and 841bp). Lane 4, the right size of full-length of SR-PSOX fragment was amplified after PCR reaction. M, DNA ladder.