Figure 2
From: Function modification of SR-PSOX by point mutations of basic amino acids
The transfected 293T cells were expressing pEGFP and SR-PSOX proteins detected by confocal microscopy. 293T cells were transiently transfected with pEGFP-N3 empty vector or pEGFP-N3-SR-PSOX construct (wild-type SR-PSOX cloned into pEGFP-N3 vector) for 24 hours respectively. The fluorescence images of the cells were observed under confocal microscope after the cells were immunostained with APC-labeled rat anti-human SR-PSOX antibody. Top panel (vector), the cells were transfected with pEGFP-N3 empty vector alone. Bottom panel (wild type), the cells were transfected with pEGFP-N3-SR-PSOX construct.