Expression levels of SR-PSOX on the surface of transfected 293T cells. 293T cells were transiently transfected with pEGFP-N3 empty vector, wild-type SR-PSOX and different mutant constructs respectively. After 24 hours transfection, the cells were harvested and stained with APC-labeled anti-SR-PSOX antibody. The expression levels of SR-POSX on the surface of the cells in each group were measured by FACS. The data are represented as the mean ± SEM by the percentage of SR-PSOX-positive cells in the EGFP-positive cells from three independent experiments.