Figure 5
From: Function modification of SR-PSOX by point mutations of basic amino acids
Phagocytosis of bacteria by the transfected 293T cells. 293T cells were transiently transfected with pEGFP-N3 empty vector, wild-type SR-PSOX and different mutant constructs respectively. After 24 hours transfection, the cells were incubated with Alexa Fluor 594-labeled E. coli for 4 hours at 37°C. The mean values of phagoctosed bacterial are represented as the intensity of Alexa Fluor 594 fluorescence in the EGFP-positive 293T cells. The values are relative to the wild-type SR-PSOX group set as 100%. The data show the mean ± SEM from four independent experiments. *: P < 0.05, **: P < 0.01, each compared to wild-type SR-PSOX (WT).