Figure 1
From: A novel AMPK activator, WS070117, improves lipid metabolism discords in hamsters and HepG2 cells
A novel structure compound, WS070117, activated AMPK in HepG2 cells. A: Structure of WS070117; B: Effect of WS070117 on AMPK activity in HepG2 cells detected by AMPK activity assays with SAMS peptide and [γ-32P]ATP used as substrates. HepG2 cells were treated with WS070117 (10 μM) for 1h, 6h and 12h. C: Effect of 12h treatment of WS070117 (1,10,100 μM) or AICAR (1 mM) on AMPK activity of HepG2 cells. AMPK activities are expressed relative to activity detected in HepG2 cells lysate. Each point is the mean (± SEM) of 3 separate experiments. **P < 0.01, *P < 0.05 as compared with control.