Figure 3

the activation of SPL by colipases from other species and the capacity of CoSPL to activate various pancreatic lipases. (A) Effect of varying amounts of stingray, dromedary, turkey and chicken pancreatic colipases on the activity of pure SPL in the presence bile salts at pH 8.5 and 37°C. Enzymatic activity of lipase (2.1 nM) was assayed in the presence of 6 mM NaTDC with increasing amounts of colipase. (open circle): CoCPL; (open square): CoDrPL; (black square): CoSPL; (black circle): CoTPL (B) Effect of varying amounts of stingray pancreatic colipase on the activity of pure lipases from various species in the presence bile salts at pH 8.5 and 37°C. Enzymatic activity of lipase (2.1 nM) was assayed in the presence of 6 mM NaTDC with increasing amounts of colipase. (open circle): CoCPL; (open square): CoDrPL; (black square): CoSPL; (black circle): CoTPL