Figure 4

Chromatography of stingray colipase on FPLC Mono-S Sepharose and FPLC Mono-Q Sepharose. (A) Chromatography of stingray pancreatic colipase on FPLC Mono-S Sepharose. The column (2.6 cm × 20 cm) was equilibrated with 100 mM acetate buffer, pH 4.5, containing 0.05% Triton X-100 and 2 mM benzamidine (buffer A); a linear salt gradient (0.1 to 0.4 M NaCl) in buffer A was applied to the column; gradient chamber 200 ml; 3 ml fraction; flow rate, 30 ml/h. (B) Chromatography of stingray pancreatic colipase on FPLC Mono Q Sepharose step. The column (2.6 cm × 20 cm) was equilibrated with 10 mM tris-HCl buffer, pH 8, containing 10 mM NaCl and 2 mM benzamidine (buffer B); a linear gradient (0.1 to 0.3 M NaCl) in buffer B was applied to the column; 3 ml fraction; flow rate, 30 ml/h.