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Table 1 Flow sheet of stingray pancreatic colipase purification

From: Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca

Purification step Total(a)activity (units) Protein(b)(mg) Specific activity (U/mg) Activity recovery (%) Purification factor
Heat and acidic treatment 78000 500 156 100 1
(NH 4 ) 2 SO 4 Precipitation (50%) 62400 200 312 80 2
Ethanol fractionation (50-90%) 48672 50 973.4 62.4 6.24
Mono S Sepharose 36500 15.5 2354.8 46.8 15.1
Mono Q Sepharose 27400 2.98 9200 35.1 59
  1. (a)1 Unit: One colipase unit corresponds to the amount of the cofactor that increases bile salts inhibited pancreatic lipase activity by one enzyme unit.
  2. (b)Proteins were estimated by Bradford method [24]. The experiments were conducted three times.