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Table 1 Flow sheet of stingray pancreatic colipase purification

From: Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca

Purification step

Total(a)activity (units)

Protein(b)(mg)

Specific activity (U/mg)

Activity recovery (%)

Purification factor

Heat and acidic treatment

78000

500

156

100

1

(NH 4 ) 2 SO 4 Precipitation (50%)

62400

200

312

80

2

Ethanol fractionation (50-90%)

48672

50

973.4

62.4

6.24

Mono S Sepharose

36500

15.5

2354.8

46.8

15.1

Mono Q Sepharose

27400

2.98

9200

35.1

59

  1. (a)1 Unit: One colipase unit corresponds to the amount of the cofactor that increases bile salts inhibited pancreatic lipase activity by one enzyme unit.
  2. (b)Proteins were estimated by Bradford method [24]. The experiments were conducted three times.
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Lipids in Health and Disease

ISSN: 1476-511X