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Table 4 Kinetic parameters between stingray colipase and lipases of various species deduced from figure 4B

From: Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca

  apparent Kd (10-9M) apparent Vmax (μmol min-1mg-1) kcat (S-1) kcat/Kd (S-1M-110-9)
SPL 1.15 ± 0.05 3200 ± 102 2400 ± 95 2074 ± 107
TPL 2.9 ± 0.1 3655 ± 85 2741 ± 150 946 ± 48
CPL 4.5 ± 0.5 3428 ± 132 2747 ± 87 611 ± 30
DrPL 3 ± 0.2 3341 ± 135 2785 ± 135 930 ± 71
  1. The apparent Kd were deduced from the slope of the linear curves of the Scatchard plots. The experiments were conducted three times.