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Table 3 LC-MS-MS analysis of sphingolipid composition in total serum fractions from CCHS serum samples.

From: S1P, dihydro-S1P and C24:1-ceramide levels in the HDL-containing fraction of serum inversely correlate with occurrence of ischemic heart disease

Sphingolipid1 Concentration (nM)
  High HDL-C, IHD2 High HDL-C, No IHD2   Low HDL-C, IHD2 Low HDL-C, No IHD2  
  Mean Std Mean Std P value 3 Mean Std Mean Std P value 3
S1P 1889.9 546.0 1849.8 407.6 O-NS 1879.2 549.0 2053.0 628.0 O-NS
Sph BDL 22.3 11.0 NA 16.0 11.6 52.3 59.9 <0.0001
DHSph BDL 14.8 9.2 NA 10.1 5.1 16.6 11.6 <0.0001
DH-S1P 213.0 76.1 276.8 96.5 0.0032 273.9 98.2 271.3 106.0 0.9992
C16-Cer 171.4 70.7 212.0 86.8 0.0276 360.1 163.9 222.5 78.7 <0.0001
DHC16-Cer 139.1 74.9 60.1 38.5 <0.0001 77.1 39.7 75.5 31.6 0.9958
C14-Cer 54.6 8.9 92.3 19.7 <0.0001 117.0 34.4 103.0 35.1 0.0412
C-18:1-Cer 91.6 33.3 49.5 13.5 <0.0001 63.7 25.6 65.2 13.6 0.5492
C18-Cer 163.8 51.0 123.9 42.3 0.0002 171.2 69.9 123.2 31.9 <0.0001
C20:1-Cer 57.5 38.5 17.4 6.1 <0.0001 28.4 15.4 25.0 9.7 0.9997
C20-Cer 481.1 205.9 249.9 94.2 <0.0001 320.9 153.3 270.8 120.0 0.3547
C22-Cer 1045.2 347.4 758.2 194.0 <0.0001 1116.0 420.3 915.4 317.9 0.0147
C20:4-Cer BDL BDL NA BDL 6.7 10.1 NA
C22:1-Cer 128.7 118.9 62.3 37.3 0.0061 206.5 160.1 61.4 47.9 <0.0001
C24-Cer 6026.3 1816.2 5522.0 1381.9 O-NS 5577.5 2080.1 5724.1 1715.1 O-NS
C24:1-Cer 3433.4 847.9 2424.3 545.8 <0.0001 2838.6 951.2 3223.2 688.7 0.0681
C26-Cer 40.7 32.8 33.8 15.7 O-NS 38.9 30.2 36.0 18.8 O-NS
C26:1-Cer 30.7 20.0 30.8 11.9 0.8093 40.3 21.2 29.8 15.9 0.0140
  1. 1Abbrevations used: Sph, sphingosine; DH, dihydro; Cer, ceramide; P, phosphate; BDL, below detection limit; O-NS, Overall not significant by ANOVA; NA, not applicable since at least one group in the pair was below detection limit.
  2. 2Sample sizes are High HDL-C, IHD, n = 53; High HDL-C, no IHD, n = 55; Low HDL-C, IHD, n = 42; Low HDL-C, no IHD, n = 54.
  3. 3p-values are derived from a Tukey's comparison following a one-way ANOVA test conducted at level of significance 0.05.