Figure 3
From: Palmitic and linoleic acids induce ER stress and apoptosis in hepatoma cells
Effects of palmitic and linoleic acids on the expression of CHOP, GRP78, and GRP94 in protein level. The Rat liver tumor cells were treated with TG, PA(250 μM), LA (250 μM) alone or PA+LA (125 μM or 250 μM) for 16 hr. The cells were treated with PBS as Control group. The levels of the respective proteins were determined by Western blot and normalized to the level of β-actin (n = 3 in each group). The relative expression of protein (A) CHOP, (B) GRP78 and (C) GRP94 were summarized and representative western blot photos were presented in (D). a: P < 0.05 compared to Control; b: P < 0.05 compared to PA; c: P < 0.05 compared to TG after Bonferroni adjustment. In (D), lane 1 and 5 were loaded with Control, lane 2 for TG, lane 3 and 6 for PA, lane 4 for LA, lane 7 for PA+LA (125 μM) and lane 8 for PA+LA (250 μM).