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Table 1 Primers for quantitative RT-PCR

From: 4,8-Sphingadienine and 4-hydroxy-8-sphingenine activate ceramide production in the skin

Gene

Primer sequence (Forward)

Primer sequence (Reverse)

Reference

SPTLC2

CCAGACTGTCAGGAGCAACCATTA

CGTGTCCGAGGCTGACCATA

 

CerS2

CCGATTACCTGCTGGAGTCAG

GGCGAAGACGATGAAGATGTTG

[12]

CerS3

ACATTCCACAAGGCAACCATTG

CTCTTGATTCCGCCGACTCC

[12]

CerS4

CTTCGTGGCGGTCATCCTG

TGTAACAGCAGCACCAGAGAG

[12]

CerS5

GCCATCGGAATCAGGAC

GCCAGCACTGTCGGATGT

[12]

CerS6

GGGATCTTAGCCTGGTTCTGG

GCCTCCTCCGTGTTCTTCAG

[12]

DEGS-1

GCGTTTGGCAGTTGCATTAA

CATTGTGGGCAATCTCATGAA

designed by Primer Express

SMS-1

GCCAGGACTTGATCAACCTAACC

CCATTGGCATGGCCGTTCTTG

[13]

SMS-2

CACCCAGTGGCTGTTTCTGA

TGCATTCCAGGCACAGGTAGA

[13]

aSMase

TGGCTCTATGAAGCGATGGC

TTGAGAGAGATGAGGCGGAGAC

[14]

β-GCS

GCTAGGCTCCTGGGATCGAG

GTTCAGGGCAAGGTTCCAGTC

[15]

GCS

GTTCGTCCTCTTCTTGGTGC

AGAAGAGAGACACCTGGGAGC

[16]

ELOVL-1

ATTCTCCTGACCTACGTGTACTT

TTCCGATTAGCCATGATGCGA

Primer Bank

ELOVL-4

CATGTGTATCATCACTGTACG

AAAGGAATTCAACTGGGCTC

[17]

ELOVL-5

TAACAGGAGTATGGGAAGGCA

ACCAGAGGACACGGATAATCTT

Primer Bank

ELOVL-6

TGCTGCCTTTATATTCGGTGG

CCTCAGTTCAAACTTTGCTCGTT

Primer Bank

ELOVL-7

TTCCATCATACCATCATGCC

CCCAATGCAGAAAGTCCATA

[18]

PPARβ/δ

ACAGCATGCACTTCCTTCCA

TCACATGCATGAACACCGTA

[19]

PPARγ

ATTCTGGCCCACCAACTTTG

TCCATTACGGAGAGATCCACG

[20]

GAPDH

TGCACCACCAACTGCTTAGC

GGCATGGACTGTGGTCATGAG

[21]

  1. Primer sequences used in real-time quantitative RT-PCR in this study. All primers were used with SYBR-Green and their specificities checked using the melting curve method (60–95°C).