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Figure 3 | Lipids in Health and Disease

Figure 3

From: Toxicity of oxidized phospholipids in cultured macrophages

Figure 3

Time-dependent stability of oxPL in culture media under different serum conditions. OxPL were incubated in DMEM containing 0,1% or 10% serum for different time periods. Subsequently, lipids were isolated by solvent extraction and separated by TLC. Mobile phase for PGPC (Rf = 0,43) and E-PGPC (Rf = 0,36), POPC (Rf >0,30) and PLPC (Rf = 0,18) was chloroform/methanol/acetone/glacial acetic acid/water 20/40/10/10/10 (v/v/v/v/v). For POVPC (Rf = 0,35) and E-POVPC (Rf = 0,32), POPC (Rf >0,25) and PLPC (Rf = 0,15) the mobile phase was chloroform/methanol/water 50/30/10 (v/v/v) Under high serum conditions (10% FCS), POVPC and PGPC were converted to lysophospholipids. Under low serum conditions (0,1% FCS), PGPC was stable for at least 6 h, whereas POVPC started getting degraded immediately (Panel A). Notably, the major amount of oxPL stayed intact during the incubation times used for cell experiments. The 1-O-alkyl ether analogs showed the same stabilities as their acyl counterparts (Panel B).

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