Hepatic gene expression involved in cholesterol metabolism. KK-Ay mice were fed with the AIN-93G diet with or without 0.2% fucoxanthin for 4 weeks. Total RNA was extracted from the liver and processed for quantitative real-time PCR analysis as described under “Materials and Methods”. The expression levels of each mRNA are normalized to β-actin levels. Data are presented relative to the control group. Each value represents the mean ± standard error (n = 6). HMGCR: HMG-CoA reductase, HMGCS: HMG-CoA synthase, FDPS: farnesyl diphosphate synthase, LSS: lanosterol synthase, CYP51: lanosterol 14α-demethylase, CYP7A1: cholesterol 7 α-hydroxyrase.