Localisation of sphingomyelinases in subcutaneous adipose tissue. Representative pictures of immunohistochemical staining of macrophages and multinuclear giant cells (aggregated macrophages) (A-D) and a small blood vessel (E-J) within subcutaneous adipose tissue from an obese woman. Brown coloration indicates macrophage-specific CD68 (A and E), SMPD1 (B and F), SMPD2 (C and G), SMPD3 (D and H) and PECAM-1 (I). All sections were counterstained with haematoxylin (coloured blue). Collagen staining (J) was visualized with polarized light to reveal mature, extracellular collagen (coloured red).