Skip to main content

Table 2 Expression in subcutaneous adipose tissue of ceramide-metabolising enzymes in relation to adipose tissue inflammation

From: Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue

Gene ABI assay ID Ct value Less inflamed adipose tissue, normal liver fat More inflamed adipose tissue, high liver fat P
    n = 10 n = 10  
SPTLC1 Hs00272311_m1 27.2 0.99 ± 0.04 1.05 ± 0.06 0.20
SPTLC2 Hs00191585_m1 26.8 0.97 ± 0.07 1.00 ± 0.05 0.39
DEGS1 Hs00186447_m1 25.9 0.99 ± 0.05 1.07 ± 0.05 0.11
LASS1 Hs00242151_m1 33.2 9.07 ± 3.12 9.22 ± 1.63 0.48
LASS4 Hs00226114_m1 27.1 1.08 ± 0.10 1.07 ± 0.16 0.49
LASS6 Hs00826756_m1 28.8 0.87 ± 0.07 0.89 ± 0.07 0.42
ASAH1 Hs00602774_m1 24.3 1.16 ± 0.10 1.47 ± 0.13 0.03
UGCG Hs00234293_m1 28.2 0.91 ± 0.08 0.96 ± 0.06 0.34
SGMS1 Hs00380453_m1 28.0 1.01 ± 0.05 1.10 ± 0.06 0.12
SGMS2 Hs00398067_m1 30.6 2.09 ± 0.27 2.57 ± 0.32 0.13
SMPD1 Hs00609415_m1 26.6 1.51 ± 0.10 1.85 ± 0.10 0.01 a
SMPD2 Hs00162006_m1 29.8 1.13 ± 0.06 1.26 ± 0.06 0.08 a
SMPD3 Hs00218713_m1 33.8 1.33 ± 0.13 1.76 ± 0.18 0.05 a
SMPD4 Hs00215775_m1 29.1 1.57 ± 0.09 1.52 ± 0.09 0.35
CERK Hs00368483_m1 29.3 1.57 ± 0.15 1.87 ± 0.10 0.06
SPHK1 Hs00184211_m1 28.9 12.18 ± 1.49 17.64 ± 1.65 0.01
CGT Hs00409961_m1 35.5 4.45 ± 1.29 3.45 ± 0.52 0.24
CDH5 Hs00174344_m1 28.3 1.16 ± 0.14 1.43 ± 0.13 0.09
SELE Hs00950401_m1 33.2 0.72 ± 0.15 2.45 ± 0.94 0.04
VEGF Hs0090054_m1 26.4 4.13 ± 0.37 3.71 ± 0.52 0.25
HIF-1α Hs00153153_m1 27.6 1.39 ± 0.12 1.65 ± 0.08 0.02
  1. Relative gene expression levels of genes involved in ceramide and sphingomyelin metabolism in subcutaneous adipose tissue of obese women with different degrees of adipose tissue inflammation are given. SPTLC: serine palmitoyl transferase long-chain; DEGS: dihydroceramide desaturase; LASS: LAG1 homolog (ceramide synthase); ASAH: N-acylsphingosine amidohydrolase (ceramidase); UGCG: UDP-glucose ceramide glucosyltransferase; SGMS: sphingomyelin synthase; SMPD: sphingomyelin phosphodiesterase (sphingomyelinase); CERK: ceramide kinase; SPHK: sphingosine kinase; CGT: ceramide glycosyl transferase; CDH5: cadherin 5 (vascular endothelium); SELE: selectin E; VEGF: vascular endothelial growth factor; HIF-1α: hypoxia-inducible factor 1α. Gene expression was normalized to housekeeping genes RPLP0 and TBP, which did not differ between the groups (for RPLP0: 1.94 ± 0.26 vs 2.11 ± 0.17, NS; for TBP: 3.96 ± 0.48 vs 3.97 ± 0.44, NS). Values are expressed as mean ± SEM. a Reported previously [3].