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Table 2 Identification results of proteins differentially expressed in basal and after ω-3 PUFAs supplementation

From: Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome

Spot

Protein name

ANOVA (p < 0.05)

Fold change

Protein expression level after ω-3 supplementation

UniProt ID

Entry name

Mascot score

Sequence coverage (%)

Number of Peptidic masses identified

Number of MS/MS identified

MW (KDa)

pI

837

Apolipoprotein A-I

0.044

1.18

Up-regulated

P02647

APOA1

87

42

10

1

30.8

5.56

997

Clusterin

0.029

1.3

Up-regulated

P10909

CLUS

735

47

30

8

53

5.89

610

Fibrinogen β

0.048

1.1

Up-regulated

P02675

FIBB

233

49

29

4

56.6

8.54

612

Fibrinogen β

0.045

1.1

Up-regulated

P02675

FIBB

140

45

20

1

56.6

8.54

721*

Clusterin

0.02

1.4

Up-regulated

P10909

CLUS

519

23

 

11

53

5.89

Apolipoprotein A-I

0.02

1.4

Up-regulated

P02647

APOA1

478

39

 

13

30.8

5.56

Haptoglobin-related protein

0.02

1.4

Up-regulated

P00739

HPTR

477

26

 

12

39.5

6.63

732

Haptoglobin-related protein

0.015

1.37

Up-regulated

P00739

HPTR

79

31

14

4

39.5

6.63

965

Serum paraoxonase

0.003

1.6

Up-regulated

P27169

PON1

142

15

9

4

39.8

5.08

970

Serum paraoxonase

0.003

1.97

Up-regulated

P27169

PON1

411

56

24

8

39.8

5.08

973

Serum paraoxonase

0.003

1.86

Up-regulated

P27169

PON1

235

31

14

4

39.8

5.08

825*

Ig kappa chain C region

0.036

1.27

Up-regulated

P01834

IGKC

872

86

 

8

11.6

5.87

Apolipoprotein A-I

0.036

1.27

Up-regulated

P02647

APOA1

397

52

 

15

30.8

5.56

578*

α-1-antitrypsin

0.001

0.74

Donw-regulated

P01009

A1AT

364

51

 

18

46.7

5.59

986*

Complement C1r subcomponent

0.018

0.85

Donw-regulated

P00736

C1R

1601

65

 

45

80.1

6.21

987*

Serum Albumin

0.032

0.85

Donw-regulated

P02768

ALBU

1114

75

 

46

69.3

6.3

Ig mu Chain C region

0.032

0.85

Donw-regulated

P01871

IGHM

987

57

 

25

49.3

6.8

Complement C1r subcomponent

0.032

0.85

Donw-regulated

P00736

C1R

784

46

 

28

80.1

6.2

324

Complement Factor H

0.017

0.72

Donw-regulated

P08603

CFAH

72

16

19

1

14.4

6.21

980*

Fibronectin

0.01

0.66

Donw-regulated

P02751

FN1

1434

26

 

40

262.5

5.71

981*

Fibronectin

0.002

0.67

Donw-regulated

P02751

FN1

1437

30

 

48

262.5

5.71

  1. Spots label with a * denotes identification by Orbitrap. Positive fold changes denote proteins up-regulated while negative fold changes denote decrease protein expression after the supplementation. MW denotes Molecular Weight. pI denotes Isoelectric point.