Quantitation of cerebral apo B in mice randomised to diet + drug for 8 weeks. The bar graphs show 3-dimensional (3D) quantitative analysis of apo B leakage of wild-type mouse brains. Pixel intensity of apo B was quantitated surrounding the cerebrovasculature in mice fed low-fat (LF), saturated-fatty acids (SFA) and SFA mice randomised to diets containing atorvastatin (LF + At, SFA + At), pravastatin (LF + Pr, SFA + Pr) and ibuprofen (LF + Ib, SFA + Ib). Apo B pixel intensity was measured in the cortex (CTX, black column), brain-stem (BS, white column) and hippocampal formation (HPF, dotted column) and expressed as per unit volume. Symbol → indicate that mice were fed SFA diet for 12 weeks and then the diet was replaced (→) with a drug containing diet. The bars represent mean intensity and standard error of mean, where P < 0.05 considered statistically significant. a: statistically significant in comparison to LF. b: statistically significant in comparison to SFA.