Figure 2

SR-BI does not affect ABCA1-mediated cellular cholesterol efflux. CHO cells were transiently transfected with SR-BI alone, ABCA1 alone, and SR-BI plus ABCA1 (co-transfection). 24 h after transfection, cholesterol efflux was initiated by the addition of HDL3 (150 μg protein/ml) and apoA-I (10 μg/ml) to medium for 24 h. The increased FC (A), CE (B) and TC (C) mass in medium were determined. Values are means ± SD of three independent experiments. * p < 0.05, ** p < 0.01. D shows the western blots of SR-BI and ABCA1 protein in vector transfected, SR-BI transfected, ABCA1 transfected and cotransfected cells.