Effects of adiponectin on palmitate-induced apoptosis in H9c2 cells. Cells were pretreated with 2.5 μg/mL globular adiponectin for 1 h before exposure to 150 μM palmitate for 12 h. Cell apoptosis was determined by Hoechst 33342 staining (A and B), and cell viability was measured using MTT assay (C). Levels of cleaved caspase-3 (D) and PARP (E) were measured by immunoblot and quantitative analysis. Results were reported as mean ± S.E.M. * P< 0.05 versus control, # P< 0.05 versus PA.