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Figure 4 | Lipids in Health and Disease

Figure 4

From: Lipopolysaccharide promotes lipid accumulation in human adventitial fibroblasts via TLR4-NF-κB pathway

Figure 4

The induced lipid accumulation resulted from up-regulated expression of ADRP via LPS-activated TLR4 and NF-κB pathway. Cultured adventitial fibroblasts were pretreated with or without anti-TLR 4 antibodies and the NF-κB inhibitor PDTC for 1 h before exposure to 10 μg/ml of LPS for 48 h. Compared to the control group, treatment with the inhibitors significantly abrogated the LPS-induced increase in ADRP expression and lipid deposition. (A) The activation and inhibition of NF-κB pathway was analyzed by Western blotting. Cultured cells were pretreated with, or without, NF-κB inhibitor PDTC before LPS stimulation. (B) Induced lipid accumulation via the LPS-activated TLR4 and NF-κB pathway. (C) The induced lipid accumulation was accompanied with ADRP expression. The expression of ADRP mRNA in activated, or not, cells were analyzed by real-time PCR. ** P < 0.01. *P < 0.05.

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