Figure 3
From: Inhibition of uncoupling protein 2 with genipin exacerbates palmitate-induced hepatic steatosis
Tempol blocks genipin-mediated hepatic steatosis. Neutral fat accumulations after 24 h of exposure to palmitic acid (PA, 250 μmol/L) (A), PA plus genipin (5 μmol/L) (B) and PA plus genipin and tempol (1 mmol/L) (C) are shown by staining of HepG2 cells with Oil-Red O. (D) Triglyceride level normalized to cellular protein content in HepG2 was determined. Data are expressed as mean ± SEM. ** p < 0.01 compared with the PA group, #p < 0.05 compared with the cells treated with PA plus genipin. PA, palmitic acid; T, tempol.