The effect of DHT on the secretion of ApoM secretion and the levels of ApoM mRNA are mediated by PKC pathways. Cells were treated with different concentrations of PMA or Staurosporine or vehicle (control) for 24 h, the levels of ApoM mRNA were determined by RT-PCR (A,B). Cells were treated with 5 μM PMA or 50nM Staurosporine or vehicle for 24 h, ApoM concentrations were determined by western blotting analysis (C). Cells were incubated in 1 μM DHT for 24 h in present different concentrations of Staurosporine or absent Staurosporine (control). ApoM concentrations were determined by western blotting analysis (D), Lane 1, control group(1 μM DHT without Staurosporine), lanes 2–5, 1 μM DHT with Staurosporine concentrations of 1,5, 50, and 100 nM respectively. The levels of ApoM mRNA were determined by RT-PCR as described in “Materials and methods.” Data are expressed relative to the control group (100%). Data are represented as means (SD) (n = 6 for each sample group). *, P < 0.05 vs. control group. ***, P < 0.01 vs. control group.