Figure 2From: E series prostaglandins alter the proliferative, apoptotic and migratory properties of T98G human glioma cells in vitroEffect of IBP on cell number, mitotic index and bromodeoxyuridine (BrdU) incorporation in T98G cells. (A) IBP decreased cell number after 72 h at both 25 and 50 μM. Statistical significance, *p = 0.0010 and **p = 0.0005, n = 3. (B) The number of cells in mitosis was obtained by Hoechst 33342 staining, and counting at x200 magnification. Statistical significance, *p = 0.0001, n = 8. (C) The nuclear incorporation of BrdU in the S-phase was measured after DAB staining and methyl green counterstaining. Statistical significance, *p < 0.0011, n = 4. Each experiment was repeated at least three times.Back to article page