Figure 6
Effect of PGE 1 and PGE 2 on cell number, mitotic index, bromodeoxyuridine (BrdU) incorporation and apoptosis in T98G cells. (A) Both PGE1 and PGE2 increased cell number at 10 μM after 72 h treatment. Statistical significance *p = 0.0007 and **p = 0.0008, n = 4. (B) The number of cells in mitosis was obtained by Hoechst 33342 staining, and counting at x200 magnification. n = 8. (C) The nuclear incorporation of BrdU in the S-phase was measured after DAB staining and methyl green counterstaining. Statistical significance *p < 0.0001, n = 4. (D) Morphological changes in the nuclei typical of apoptosis were analysed after staining with Hoechst 33342. Each experiment was repeated at least three times.