Skip to main content
Figure 3 | Lipids in Health and Disease

Figure 3

From: Fatty acids do not pay the toll: effect of SFA and PUFA on human adipose tissue and mature adipocytes inflammation

Figure 3

Anti-inflammatory effects of PUFA on human adipose tissue and adipocytes. Adipose tissue (A, C, E) and adipocytes (B, D, F) were treated with 1 μg/ml LPS with or without PUFA (EPA, DHA and OA) at the concentration of 5 and 10 μM. A and B: IL-6 secretion was measured in media by ELISA after 12 hours treatment. Results were expressed in arbitrary units, normalised to LPS (1 represents from 0.3 to 0.5 μg/ml IL6 - adipose tissue, and 0.05 to 0.1 μg/ml - adipocytes, depending on the patient). C and D: TNF-alpha secretion was measured in media by ELISA after 6 hours treatment. Results were expressed in arbitrary units, normalised to LPS (1 represents from 3 to 5 ng/ml TNF-alpha - adipose tissue, and 0.5 to 1 ng/ml - adipocytes, depending on the patient). E and F: MCP-1 secretion was measured in media by ELISA after 12 hours treatment. Results were expressed in arbitrary units, normalised to LPS (1 represents from 0.1 to 0.25 μg/ml MCP-1 - adipose tissue, and 0.08 to 0.14 μg/ml - adipocytes, depending on the patient). The graphs show the mean ±SD of the results from 8 patients (n=6 for each condition, for each patient).

Back to article page