Figure 3

Effects of individual isomers of CLA and CLA mixture on gene and protein expressions of PPARα pathway from the heart: (A) PDK4 gene expression, (B) PPARα gene expression, (C) protein relative band intensity statistical result of PPARα and (D) PPARα protein expression. (E) GAPDH was used as internal control for western blotting analysis. Female mice were fed one of the treatment diets for 8 weeks: control, 0.5% CLA mixture, 0.2% c9, t11 CLA and 0.2% t10, c12 CLA. Different letters in each figure indicate significant difference at P < 0.05. Numbers are mean ± S.E. (n = 5 for gene expressions and n = 3 for protein expression).