Table 2 Characteristics and performance data of the primers used for qPCR analysis and reference gene-stability measure M
From: Dietary moderately oxidized oil activates the Nrf2 signaling pathway in the liver of pigs
Gene | Forward primer (from 5' to 3') Reverse primer (from 5' to 3') | PCR product size (bp) | NCBI GenBank | Slope | R2 | Efficiency | M value |
|---|---|---|---|---|---|---|---|
Reference genes | |||||||
ATP5G1 | CAGTCACCTTGAGCCGGGCGA TAGCGCCCCGGTGGTTTGC | 94 | NM_001025218.1 | -0.2661 | 0.9981 | 1.85 | 0.036 |
GPI | CACGAGCACCGCTCTGACCT CCACTCCGGACACGCTTGCA | 365 | NM_214330.1 | -0.2557 | 0.9964 | 1.80 | 0.034 |
RPS9 | GTCGCAAGACTTATGTGACC AGCTTAAAGACCTGGGTCTG | 327 | CAA23101 | -0.2705 | 0.9994 | 1.86 | 0.036 |
β-Actin | GACATCCGCAAGCACCTCTA ACATCTGCTGGAAGGTGGAC | 205 | NM_001167795 | -0.2637 | 0.9979 | 1.84 | 0.046 |
SDHA | CTACGCCCCCGTCGCAAAGG AGTTTGCCCCCAGGCGGTTG | 380 | DQ402993 | -0.2551 | 0.9986 | 1.80 | 0.041 |
Target genes | Â | Â | Â | Â | Â | Â | Â |
SOD1 | TCCATGTCCATCAGTTTGGA CTGCCCAAGTCATCTGGTTT | 250 | NM_001190422.1 | -0.2773 | 0.9997 | 1.89 | - |
TXNR1 | CTTTACCTTATTGCCCGGGT | 162 | NM_214154.2 | -0.2615 | 0.9998 | 1.83 | - |
| Â | GTTCACCGATTTTGTTGGCC | Â | Â | Â | Â | Â | Â |
GPX1 | CTTCGAGAAGTTCCTGGTGG CCTGGACATCAGGTGTTCCT | 232 | NM_214201.1 | -0.2594 | 0.9986 | 1.82 | - |
HO-1 | AGCTGTTTCTGAGCCTCCAA CAAGACGGAAACACGAGACA | 130 | NM_001004027.1 | -0.2828 | 0.9917 | 1.92 | - |
UGT1A1 | GATCCTTTCCTGCAACGCAT GGAAGGTCATGTGATCTGAG | 313 | XM_001927673 | -0.2395 | 0.9985 | 1.74 | - |
NQO1 | CCAGCAGCCCGGCCAATCTG AGGTCCGACACGGCGACCTC | 160 | NM_001159613.1 | -0.2756 | 0.9467 | 1.92 | - |
MGST1 | TTGGCGCGCGAATCTACCACA TCCTCGGCTCCCTTCCCACTTA | 239 | NM_214300.1 | -0.2636 | 0.9946 | 1.83 | - |