Table 2 Characteristics and performance data of the primers used for qPCR analysis and reference gene-stability measure M
Gene | Forward primer (from 5' to 3') Reverse primer (from 5' to 3') | PCR product size (bp) | NCBI GenBank | Slope | R2 | Efficiency | M value |
|---|---|---|---|---|---|---|---|
Reference genes | |||||||
ATP5G1 | CAGTCACCTTGAGCCGGGCGA TAGCGCCCCGGTGGTTTGC | 94 | NM_001025218.1 | −0.2661 | 0.9981 | 1.85 | 0.036 |
GPI | CACGAGCACCGCTCTGACCT CCACTCCGGACACGCTTGCA | 365 | NM_214330.1 | −0.2557 | 0.9964 | 1.80 | 0.034 |
RPS9 | GTCGCAAGACTTATGTGACC AGCTTAAAGACCTGGGTCTG | 327 | CAA23101 | −0.2705 | 0.9994 | 1.86 | 0.036 |
β-Actin | GACATCCGCAAGCACCTCTA ACATCTGCTGGAAGGTGGAC | 205 | NM_001167795 | −0.2637 | 0.9979 | 1.84 | 0.046 |
SDHA | CTACGCCCCCGTCGCAAAGG AGTTTGCCCCCAGGCGGTTG | 380 | DQ402993 | −0.2551 | 0.9986 | 1.80 | 0.041 |
Target genes | |||||||
FGF21 | CGATACCTCTACACGGATGA CGTTGTAGCCATCCTCAAGT | 158 | NM_001163410.1 | −0.2773 | 0.9968 | 1.88 | - |
HMGCS2 | ATGGAACGCACGCAGCTCCC TCAGGCCCAACCAGCATGGC | 323 | NM_214380.1 | −0.2528 | 0.9983 | 1.79 | - |
ACO | CTCGCAGACCCAGATGAAAT TCCAAGCCTCGAAGATGAGT | 218 | AF185048 | −0.2495 | 0.9986 | 1.78 | - |
L-CPT1 | GCATTTGTCCCATCTTTCGT GCACTGGTCCTTCTGGGATA | 198 | AF288789 | −0.2654 | 0.9986 | 1.84 | - |
OCTN2 | TGACCATATCAGTGGGCTA AGTAGGGAGACAGGATGCT | 384 | XM_003123912 | −0.2517 | 0.998 | 1.79 | - |