Figure 2

Fatty acid-induced decreases in 2DG uptake activity and viability of L6 cells.

(A) Differentiated L6 cells were incubated with the indicated fatty acid (750 μM palmitic, stearic, palmitoleic, oleic, linoleic or α-linolenic acids; or 100 μM arachidonic, docosahexaenoic or eicosapentaenoic acid) for 14 h. 2DG uptake activity was measured in the absence or presence of 100 nM insulin. (B) Cell viability was determined by crystal violet staining after treatment with the indicated concentrations of arachidonic, docosahexaenoic and eicosapentaenoic acids for 24 h. Different letters indicate significant changes (p < 0.05) for panel (A). * indicates significant difference vs control at p < 0.05 for panel (B)