RSV increases CYP7A1 expression and activity in HFD-fed mice. After 8 wks of feeding, the liver microsomes of mice were isolated. (A) The CYP7a1 mRNA and (B) protein abundance were quantified as described in the Materials and Methods section. (C) Quantification of Western blot data. *P < 0.05, n = 3. (D) CYP7A1 activity levels were expressed as pM/min/mg protein. Values are means ± SD, n = 6. Asterisks, significantly different from HFD-fed mice ( P < 0.05).