Figure 3

Inhibition of induced glucose release by FAs. Primary hepatocytes were cultured and pre-treated with FAs mixture (2 : 1 oleate:palmitate with 1% BSA) to final concentration of 1 mM FAs for the indicated times. After pre-treatments, cells were treated with the gluconeogenic inducers glucagon and cAMP (A) or dexamethasone and cAMP (B) for 6 hours. Glucose output was determined by glucose concentration measured in medium, which initially contained gluconeogenic precursors but no glucose. Glucose levels were normalized per million cells and are expressed as means ± SD. n = 3; Comparisons were performed using Tukey’s honestly significant differences (HSD) test. *P < .05 vs basal output level; ‡P < .05 vs cells pre-treated for 1 hour with FAs and then treated with gluconeogenic inducers.